Next Generation Sequencing Workshop
School of Biological Sciences, IPM
April 13, 2016
1395،فروردین 25

Introduction to NGS platforms, data formats and quality control

Learning objectives:

• Develop an understanding of NGS platforms
• Introduction to workflows
• NGS data formats
• QC and pre-processing of raw data

De-novo Genome Assembly using Velvet

Learning objectives:

• Pre-processing of read data
• De-novo genome assembly using Velvet
• Assessment and optimisation of Velvet parameters
• Assessment of assembly quality

Aligning reads to a reference and Variant Calling

Learning objectives:

• Alignment to reference genome using BWA
• Visualization of aligned reads
• Quality control and refinement of the aligned reads
• Variant calling and filtering using samtools

RNA-Seq analysis

Overview of 1000 Genome Project and beyond